منابع مشابه
Broad-Host-Range Plasmid by Electroporation
Methylotrophic bacteria able to grow on one-carbon compounds, such as methanol and monomethylamine, as sole carbon sources have received considerable attention because of their potential commercial applications, including the production of single-cell protein, amino acids, pyrroloquinoline quinone, and poly-3-hydroxybutyrate. The utility of methylotrophs should be increased by the use of recomb...
متن کاملAdaptive plasmid evolution results in host-range expansion of a broad-host-range plasmid.
Little is known about the range of hosts in which broad-host-range (BHR) plasmids can persist in the absence of selection for plasmid-encoded traits, and whether this "long-term host range" can evolve over time. Previously, the BHR multidrug resistance plasmid pB10 was shown to be highly unstable in Stenotrophomonas maltophilia P21 and Pseudomonas putida H2. To investigate whether this plasmid ...
متن کاملThe broad host range plasmid pLF1311 from Lactobacillus fermentum VKM1311.
The complete nucleotide sequence (2389 bp) of the cryptic plasmid pLF1311 from Lactobacillus fermentum VKM1311 was determined. DNA sequence analysis revealed the putative coding regions for a replicative protein (RepB), its repressor (RepA) and double-stranded (dso) and single-stranded (sso) origins. pLF1311 belongs to the pE194 family of rolling circle-replicating plasmids. A derivative of pLF...
متن کاملMobilization function of the pBHR1 plasmid, a derivative of the broad-host-range plasmid pBBR1.
The pBHR1 plasmid is a derivative of the small (2.6-kb), mobilizable broad-host-range plasmid pBBR1, which was isolated from the gram-negative bacterium Bordetella bronchiseptica (R. Antoine and C. Locht, Mol. Microbiol. 6:1785-1799, 1992). Plasmid pBBR1 consists of two functional cassettes and presents sequence similarities with the transfer origins of several plasmids and mobilizable transpos...
متن کاملRole of the parCBA operon of the broad-host-range plasmid RK2 in stable plasmid maintenance.
The par region of the stably maintained broad-host-range plasmid RK2 is organized as two divergent operons, parCBA and parDE, and a cis-acting site. parDE encodes a postsegregational killing system, and parCBA encodes a resolvase (ParA), a nuclease (ParB), and a protein of unknown function (ParC). The present study was undertaken to further delineate the role of the parCBA region in the stable ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1978
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.135.1.278-280.1978